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Open (1ig8) and closed (1hkg) forms of hexokinase from Saccharomyces cerevisiae

The open form allows binding of glucopyranose and ATP while the closed form excludes water from the active site in preparation for the catalytic reaction.

• What structural elements are present in each molecule?
• What is the subunit composition?
• What gross comparisons can be made with the two structures?
• Which residues participate in the phosphorylation reaction?
• Which residues form a hydrophobic channel?

Kuser, P. R., S. Krauchenco, O. A. Antunes, and I. Polikarpov. 2000. The high resolution crystal structure of yeast hexokinase Pii with the correct primary sequence provides new insights into its mechanism of action. J. Biol. Chem. 275:20814-20821.

Steitz, T. A., M. Shoham, and W. S. Bennett Jr. 1981. Structural dynamics of yeast hexokinase during catalysis. Philos. Trans. R. Soc. Lond. B Biol. Sci. 293:43-52.

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